Innovative Methodology Three-dimensional localization and quantification of PAF-induced gap formation in intact venular microvessels
نویسندگان
چکیده
Yanyan Jiang,* Ke Wen,* Xueping Zhou,* Diane Schwegler-Berry, Vince Castranova, and Pingnian He Department of Physiology and Pharmacology, West Virginia University, Morgantown, West Virginia; Department of Pharmacology, Tianjin Medical University, Tianjin, China; and Pathology and Physiology Research Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, West Virginia
منابع مشابه
Three-dimensional localization and quantification of PAF-induced gap formation in intact venular microvessels.
Combining single-vessel perfusion technique with confocal microscopy, this study presents a new approach that allows three-dimensional visualization and quantification of endothelial gaps under experimental conditions identical to those used to measure permeability coefficients, endothelial calcium concentration, and nitric oxide production in individually perfused intact microvessels. This app...
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Zhu, Longkun, and Pingnian He. Platelet-activating factor increases endothelial [Ca ]i and NO production in individually perfused intact microvessels. Am J Physiol Heart Circ Physiol 288: H2869–H2877, 2005. First published January 21, 2005; doi:10.1152/ajpheart.01080.2004.—We have demonstrated that inhibition of NO synthase (NOS) in endothelial cells by either the NOS inhibitor N -monomethyl-L-...
متن کاملPlatelet-activating factor increases endothelial [Ca2+]i and NO production in individually perfused intact microvessels.
We have demonstrated that inhibition of NO synthase (NOS) in endothelial cells by either the NOS inhibitor N(omega)-monomethyl-l-arginine (l-NMMA) or the internalization of caveolin-1 scaffolding domain attenuated platelet-activating factor (PAF)-induced increases in microvessel permeability (Am J Physiol Heart Circ Physiol 286: H195-H201, 2004) indicating the involvement of an NO-dependent sig...
متن کاملSphingosine-1-phosphate modulation of basal permeability and acute inflammatory responses in rat venular microvessels.
AIMS Although several cultured endothelial cell studies indicate that sphingosine-1-phosphate (S1P), via GTPase Rac1 activation, enhances endothelial barriers, very few in situ studies have been published. We aimed to further investigate the mechanisms whereby S1P modulates both baseline and increased permeability in intact microvessels. METHODS AND RESULTS We measured attenuation by S1P of p...
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We tested the hypothesis that acutely induced hyperpermeability is dependent on actin-myosin contractility by using individually perfused mesentery venules of pentobarbital-anesthetized rats. Venule hydraulic conductivity (Lp) was measured to monitor hyperpermeability response to the platelet-activating factor (PAF) 1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine or bradykinin. Perfusion wit...
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